Characterization of marine worm digestive fluid surfactants by electrospray MS/MS

TitleCharacterization of marine worm digestive fluid surfactants by electrospray MS/MS
Publication TypeConference Paper
Year of Publication2002
AuthorsP Ferguson, L, Ahrens, MJ, Rieger, RA, Cotham, WE, Brownawell, BJ, Iden, CR
Conference NameProceedings 50th Asms Conference on Mass Spectrometry and Allied Topics
Date Published12/2002
Abstract

The electrospray MS/MS was used for the qualitative characterization of digestive surfactants in a marine deposit-feeding worm. The digestive fluids collected from Neresis succinea were separated by gradient HPLC, with collection of discrete two-minute fractions and simultaneous ESI-MS, micromass and UV detection throughout 80 minutes run. Accurate mass measurement and high resolution MS/MS spectra were obtained for several surfactant candidate compounds. The compounds were identified as amide-linked conjugates of cysteine and methylcysteine with C9 to C12 mono- and poly-unsaturated fatty acids.